Application of machine learning for multi-community COVID-19 outbreak predictions with wastewater surveillance.

The potential of wastewater-based epidemiology (WBE) as a surveillance and early warning tool for the COVID-19 outbreak has been demonstrated. For areas with limited testing capacity, wastewater surveillance can provide information on the disease dynamic at a community level. A predictive model is a key to generating quantitative estimates of the infected population. Modeling longitudinal wastewater data can be challenging as biomarkers in wastewater are susceptible to variations caused by multiple factors associated with the wastewater matrix and the sewersheds characteristics. As WBE is an emerging trend, the model should be able to address the uncertainties of wastewater from different sewersheds. We proposed exploiting machine learning and deep learning techniques, which are supported by the growing WBE data. In this article, we reviewed the existing predictive models, among which the emerging machine learning/deep learning models showed great potential. However, most models are built for individual sewersheds with few features extracted from the wastewater. To fulfill the research gap, we compared different time-series and non-time-series models for their short-term predictive performance of COVID-19 cases in 9 diverse sewersheds. The time-series models, long short-term memory (LSTM) and Prophet, outcompeted the non-time-series models. Besides viral (SARS-CoV-2) loads and location identity, domain-specific features like biochemical parameters of wastewater, geographical parameters of the sewersheds, and some socioeconomic parameters of the communities can contribute to the models. With proper feature engineering and hyperparameter tuning, we believe machine learning models like LSTM can be a feasible solution for the COVID-19 trend prediction via WBE. Overall, this is a proof-of-concept study on the application of machine learning in COVID-19 WBE. Future studies are needed to deploy and maintain the model in more real-world applications.

Socioeconomic status correlations with confirmed COVID-19 cases and SARS-CoV-2 wastewater concentrations in small-medium sized communities.

Over two years into the COVID-19 pandemic, it is apparent that some populations across the world are more susceptible than others to SARS-CoV-2 infection and spread. Understanding how populations with varying demographic patterns are impacted by COVID-19 may highlight which factors are most important in targeting to combat global suffering. The first objective of this study was to investigate the association of various socioeconomic status (SES) parameters and confirmed COVID-19 cases in the state of Ohio, USA. This study examines the largest and capital city of Ohio (Columbus) and various small-medium-sized communities. The second objective was to determine the relationship between SES parameters and community-level SARS-CoV-2 concentrations using municipal wastewater samples from each city's respective wastewater treatment plants from August 2020 to January 2021. SES parameters include population size, median income, poverty, race/ethnicity, education, health care access, types of COVID-19 testing sites, and social vulnerability index. Statistical analysis results show that confirmed (normalized and/or non-normalized) COVID-19 cases were negatively associated with White percentage and registered hospitals, and positively associated with registered physicians and various COVID-19 testing sites. Wastewater viral concentrations were negatively associated with poverty, and positively associated with median income, community health centers, and onsite rapid testing locations. Additional analyses conclude that population is a significant factor in determining COVID-19 cases and SARS-CoV-2 wastewater concentrations. Results indicate that community healthcare parameters relate to a negative health outcome (COVID-19) and that demographic parameters can be associated with community-level SARS-CoV-2 wastewater concentrations. As the first study that examines the association between socioeconomic parameters and SARS-CoV-2 wastewater concentrations as well as confirmed COVID-19 cases, it is apparent that social determinants have an impact in determining the health burden of small-medium sized Ohioan cities. This study design and innovative approach are scalable and applicable for endemic and pandemic surveillance across the world.

Potential environmental and health risk when returning to normal amidst COVID-19 vaccination.

Due to the SARS-CoV-2 pandemic and restricted occupancy in work and school settings, there is a heightened risk for Legionella infection. An increase of stagnation in water pipe systems with limited water usage stimulates biofilm build-up, further facilitating Legionella proliferation. Individuals can inhale infected water aerosols and develop Legionellosis that can progress into mild flu-like symptoms or severe pneumonia. While SARS-CoV-2 vaccinations have been introduced globally, there is a concern for bacterial coinfections as individuals resume normal activities. Even with new SARS-CoV-2 variants circulating, Legionella persists as a public health threat as vulnerable communities' restrictions fluctuate. Proper water monitoring and management are critical while reopening communities. This article features Legionella characteristics and novel case reports amidst the pandemic. This article encourages greater awareness for building managers to minimize water stagnancy by disinfecting water distribution systems and promotes healthcare professionals to properly diagnose other illnesses during the ongoing pandemic to reduce morbidity and mortality.